4/10/2023 0 Comments Pjet vector in serial clonerImprovement of the transient expression system for production of recombinant proteins in plants. Current Medicinal Chemistry, 26, 365–380. Transient production of recombinant pharmaceutical proteins in plants: Evolution and perspectives. Biotechnology and Bioengineering, 106, 9–17. High-level rapid production of full-size monoclonal antibodies in plants by a single-vector DNA replicon system. Current Opinion in Biotechnology, 18, 134–141. ![]() Viral vectors for the expression of proteins in plants. Gleba, Y., Klimyuk, V., & Marillonnet, S. High-yield production of authentic human growth hormone using a plant virus-based expression system. Gils, M., Kandzia, R., Marillonnet, S., Klimyuk, V., & Gleba, Y. Gene delivery into plant cells for recombinant protein production. Practical considerations for pharmaceutical antibody production in different crop systems. Recombinant plant-derived pharmaceutical proteins: Current technical and economic bottlenecks. Current Pharmaceutical Design, 19, 5471–5477. Commercial aspects of pharmaceutical protein production in plants. AgBiotechNet, 5, 1–9.įischer, R., Schillberg, S. The biosafety of molecular farming in plants. Frontiers in Plant Science, 9, 1893.Ĭommandeur, U., Twyman, R. Plant molecular farming–integration and exploitation of side streams to achieve sustainable biomanufacturing. Veterinary Immunology and Immunopathology, 128, 178–183.īuyel, J. Camelid immunoglobulins and nanobody technology. Generation and characterization of a functional nanobody against the vascular endothelial growth factor receptor-2 angiogenesis cell receptor. Treatment-related mortality with bevacizumab in cancer patients: A meta-analysis. Drug Discovery Today: Technologies, 7, 139–146. Nanobodies–from llamas to therapeutic proteins. Inhibition of angiogenesis in human endothelial cell using VEGF specific nanobody. Biochemical and Biophysical Research Communications, 333, 328–335. Bevacizumab (Avastin), a humanized anti-VEGF monoclonal antibody for cancer therapy. Proceedings of the National Academy of Sciences, 104, 18363–18370.įerrara, N., Hillan, K. VEGF trap complex formation measures production rates of VEGF, providing a biomarker for predicting efficacious angiogenic blockade. Oncogenes and tumor angiogenesis: The question of vascular ‘supply’and vascular ‘demand.’ Seminars in Cancer Biology, 2, 93–104. Advanced Pharmaceutical Bulletin, 4, 323. Expression and purification of functional human vascular endothelial growth factor-a121 the most important angiogenesis factor. Vascular endothelial growth factor receptors in the regulation of angiogenesis and lymphangiogenesis. Opinion: Angiogenesis: An organizing principle for drug discovery? Nature Reviews. ![]() Angiogenesis in cancer and other diseases. The low level of cytoplasm-targeted AntiVEGF recombinant protein might result from the degradation of improperly folded protein.Ĭarmeliet, P., & Jain, R. Our results reveal that production of biologically active and correctly processed apoplast-targeted AntiVEGF recombinant protein is possible in plant apoplast. ![]() After purification of AntiVEGF protein using aqueous two-phase system (ATPS), purified protein was analyzed with MTT assay. AntiVEGF protein accumulation in the apoplast resulted in levels of 1.2% of TSP (Total Soluble Protein) that demonstrated a two-order increase compared to the cytoplasm-targeted protein. The apoplast-targeted AntiVEGF recombinant protein was detected in infected plants by Dot-blot, western blot, and ELISA analysis. Transcription of the target gene was confirmed with RT-PCR assays. After assembling the target gene construct and cloning into the expression vector, we infected the plants with the resulting pZYMV-AntiVEGF viral vector. In this study, we direct AntiVEGF recombinant protein accumulation to cucurbit plant apoplast using a suitable signal (Pr1b) sequence. Targeting of vascular endothelial growth factor (VEGF) using AntiVEGF can be a promising approach for angiogenesis inhibition and cancer therapy.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |